Role of Dietary Pattern on the Microbial Diversity in the Gut of the Experimental MiceAuthor : Rosa J Samuel, Omana Joy, Prakash Piruthiviraj and Thamaraiselvi Kaliannan
Volume 7 No.2 July-December 2018 pp 106-109
Animal models are important for biomedical research as they help us to understand the mechanisms and functions similar to humans. Microbiological analysis is an integral part of animal studies especially when looking for gut microbiota changes. Several factors such as diet, antibiotics etc. have an impact on the composition of the gut. While many conventional and molecular methods are used for the identification of microbiota, this study aimed at evaluating the usefulness of flow cytometry for identification of bacteria based on their ability to scatter light. Fresh gut samples from mice were collected and cultured on nutrient agar followed by plating on selective media. The isolates were characterized and differentiated by biochemical tests and FACS (fluorescent activated cell sorter) analysis. The major group of isolates obtained from culture methods and biochemical characterization were identified as Proteus and Klebsiella species. Forward and side scatter analysis of the samples showed two distinct patterns in the plots and correlated with the microbiological results. From the results it was found that flow cytometry can be effectively used as method of rapid detection of microorganisms from animal samples.
Gut, Microbiota, Flow Cytometer, Rodents
 P. J. Turnbaugh, V. K. Ridaura, J. J. Faith, F. E. Rey, R. Knight and J. I. Gordon, “The effect of diet on the human gut microbiome: A Metagenomic Analysis in humanized gnotobiotic mice”, Science Translational Medicine, Vol. 1, No. 6, pp.6ra14-6ra14, 2009.
 C. De Filippo, D. Cavalieri, M. Di Paola, M. Ramazzotti, J. B. Poullet, S. Massart, S. Collini, G. Pieraccini and P. Lionetti, “Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa”, Proceedings of the National Academy of Sciences, Vol. 107, No. 33, pp. 14691-14696, 2010.
 J. G. Cappuccino and N. Sherman, Microbiology: A Laboratory Manual, 1996.
 P. D. Cani and N. M. Delzenne, “Gut microflora as a target for energy and metabolic homeostasis”, Current Opinion in Clinical Nutrition and Metabolic Care, Vol. 10, No. 6, pp. 729-734, 2007.
 S. H. Ley, O. Hamdy, V. Mohan and F. B. Hu, “Prevention and management of type 2 diabetes: dietary components and nutritional strategies”, The Lancet, Vol. 383, No. 9933, pp. 1999-2007, 2014.
 T. Clavel, M. Fallani, P. Lepage, F. Levenez, J. Mathey, V. Rochet, M. Serezat, M. Sutren, G. Henderson, C. Bennetau-Pelissero and F. Tondu, “Isoflavones and functional foods alter the dominant intestinal microbiota in postmenopausal women”, The Journal of Nutrition, Vol. 135, No. 12, pp. 2786-2792. 2005.
 I. Martínez, G. Wallace, C. Zhang, R. Legge, A.K. Benson, T. P. Carr, E. N. Moriyama and J. Walter, “Diet-induced metabolic improvements in a hamster model of hypercholesterolemia are strongly linked to alterations of the gut microbiota”, Applied and Environmental Microbiology, Vol. 75, No. 12, pp. 4175-4184, 2009.
 R. Jumpertz, D.S. Le, P.J. Turnbaugh, C. Trinidad, C. Bogardus, J.I. Gordon and J. Krakoff, “Energy-balance studies reveal associations between gut microbes, caloric load, and nutrient absorption in humans”, The American Journal of Clinical Nutrition, Vol. 94, No. 1, pp. 58-65, 2011
 A. L. Kau, P. P. Ahern, N. W. Griffin, A. L. Goodman and J. I. Gordon, “Human nutrition, the gut microbiome and the immune system”, Nature, Vol. 474, No. 7351, p. 327, 2011.
 R. Martin, A. Nauta, K. Ben Amor, L. Knippels, J. Knol and J. Garssen, “Early life: gut microbiota and immune development in infancy”, Beneficial Microbes, Vol. 1 No. 4, pp. 367-382, 2010.
 B. D. Muegge, J. Kuczynski, D. Knights, J. C. Clemente, A. González, L. Fontana, B. Henrissat, R. Knight and J. I. Gordon, “Diet drives convergence in gut microbiome functions across mammalian phylogeny and within humans”, Science, Vol. 332, No. 6032, pp. 970-974, 2011.
 M. Million, M. Maraninchi, M. Henry, F. Armougom, H. Richet, P. Carrieri, R. Valero, D. accah, B. ialettes and D. aoult, “Obesity-associated gut microbiota is enriched in Lactobacillus reuteri and depleted in Bifidobacterium animalis and Methano brevi bactersmithii”, International Journal of Obesity, Vol. 36, No. 6, p. 817, 2012.
 P. D. Cani, B. Rodrigo, C. Knauf, A. Waget, A. M. Neyrinck, N. M. Delzenne and . Burcelin, “Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice”, Diabetes, 2008.
 V. A. Gant, G. Warnes, I. Phillips and G. F. avidge, “The application of flow cytometry to the study of bacterial responses to antibiotics”, Journal of Medical Microbiology, Vol. 39, No .2, pp. 147-154, 1993.
 H. M. Davey, “Flow cytometric techniques for the detection of microorganisms”, In Advanced Flow Cytometry: Applications in Biological Research, Springer, Dordrecht, pp. 91-97, 2003.