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Home»Articles»Alpha Amylase, Alpha Glucosidase Inhibition and Profiling of Volatile Compounds of Biologically Active Extracts from Momordica cymbalaria (Hook, Fenzl) Skin and Seeds

Alpha Amylase, Alpha Glucosidase Inhibition and Profiling of Volatile Compounds of Biologically Active Extracts from Momordica cymbalaria (Hook, Fenzl) Skin and Seeds

Author : E. Abbirami, L. Dinesh Kumar, R. Guna, N. Gayathri and T. Sivasudha
Volume 8 No.1 January-March 2019 pp 67-73

Abstract

Momordica cymbalaria (Hook, Fenzl) has been used in traditional medicine for diabetes in India. This study was designed to examine, total phenolic, total flavonoid contents and in vitro anti-oxidant activity by using seven different types of solvent extracts. The biologically active extracts, ethanol extract of fruit skin (EESK) and methanol extract of seed (MESE) were evaluated for in vitro anti-diabetic activity followed by detection of bioactive metabolites by GC-MS analysis. EESK and MESE exhibited IC50 values of 185.1 μg/ml against α – amylase and 224.4 μg/ml against α – glucosidase, respectively. GC-MS profiling of skin and seed portion of the vegetable revealed the presence of 40 compounds from EESK and 29 compounds from MESE. Thus, this study reveals that EESK and MESE exhibited in vitro anti-diabetic activity and GC-MC profiling confirms the presence of anti-diabetic phytocompounds such as L-Cysteine and cis- Vaccenic acid.

Keywords

Momordica cymbalaria, Anti-Oxidant Activity, In Vitro Anti-Diabetic Activity, GC-MS

Full Text:

References

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Momordica cymbalaria (Hook, Fenzl) has been used in traditional medicine for diabetes in India. This study was designed to examine, total phenolic, total flavonoid contents and in vitro anti-oxidant activity by using seven different types of solvent extracts. The biologically active extracts, ethanol extract of fruit skin (EESK) and methanol extract of seed (MESE) were evaluated for in vitro anti-diabetic activity followed by detection of bioactive metabolites by GC-MS analysis. EESK and MESE exhibited IC50 values of 185.1 μg/ml against α – amylase and 224.4 μg/ml against α – glucosidase, respectively. GC-MS profiling of skin and seed portion of the vegetable revealed the presence of 40 compounds from EESK and 29 compounds from MESE. Thus, this study reveals that EESK and MESE exhibited in vitro anti-diabetic activity and GC-MC profiling confirms the presence of anti-diabetic phytocompounds such as L-Cysteine and cis- Vaccenic acid.

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Department of Environmental Toxicology, Texas Tech University, Texas
[email protected]
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Department of Electrical Engineering, University of Malaya, Malaysia
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Department of Mechanical Engineering, University of Akron, Akron, USA
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Dr. Mohd Faiz Bin Mohd Salleh
Department of Electrical Engineering, University of Malaya, Malaysia
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Department of Electrical Engineering, University of Malaya, Malaysia
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Department of Electrical Engineering, University of Malaya, Malaysia
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    Editorial Note

    Editorial Dr. Seshadri Ramkumar

    Editor-in-Chief
    Dr. Seshadri Ramkumar
    Department of Environmental Toxicology, Texas Tech University, Texas
    [email protected]
    Editorial Advisory Board
    Dr. Kamarul Ariffin Bin Noordin
    Department of Electrical Engineering, University of Malaya, Malaysia
    [email protected]
    Dr. Benjamin T.F. Chung
    Department of Mechanical Engineering, University of Akron, Akron, USA
    [email protected]
    Dr. Mohd Faiz Bin Mohd Salleh
    Department of Electrical Engineering, University of Malaya, Malaysia
    [email protected]
    Dr. Suhana Binti Mohd Said
    Department of Electrical Engineering, University of Malaya, Malaysia
    [email protected]
    Dr. Norrima Binti Mokhtar
    Department of Electrical Engineering, University of Malaya, Malaysia
    [email protected]
    Dr. Mohamadariff
    Department of Electrical Engineering, University of Malaya, Malaysia
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