Mathematical Modeling and Simulation of Enzyme Assisted Bioactive Compound Extraction from Allium Cepa Using Response Surface MethodologyAuthor : K. Thirugnanasambandham
Volume 7 No.1 January-June 2018 pp 43-48
In this present study, an attempt was made to investigate the enzyme assisted extraction process to extract the phenolic compounds from onion (Allium cepa) under various operating conditions such as temperature, extraction time and enzyme concentration. Three factors three level Box-Behnken response surface design (BBD) coupled with desired function methodlogy was used to optimize and model the extraction process. Optimum extracting conditions for the maximum phenloic compounds were determined and it was found to be: temperature of 40 0C, extraction time of 16 hour and enzyme concentration of 2 %. Under these conditions, 9.25 % of phenolic compound was extracted. Extracted phenolic compounds can be regarded as the promising antioxidants to scavenge the DPPH free radicals.
Phenolic compounds, Onion, Enzyme assisted extraction, Modelling, Optimzation
 S. Chen, X. H. Xing, J. J. Huang and M. S. Xu, “Enzyme-assisted extraction of flavonoids from Ginkgo Biloba leaves: improvement effect of Flavonol Transglycosylation catalyzed by Penicillium decumbens cellulose” Enzyme Microb. Techn., Vol. 48, pp. 100–105, 2011.
 Y. Chen, M. Y. Xie, S. P. Nie, C. Li and Y. X. Wang, “Purification, composition analysis and antioxidant activity of a polysaccharide from the fruiting bodies of Ganoderma atrum”, Food Chem., Vol. 107, pp. 231–241, 2008.
 J. E. N. Dolatabadi and S. Kashanian, “A review on DNA interaction with synthetic phenolic food additives”, Food Res. Int., Vol. 43, pp. 1223–1230, 2010.
 H. Falleh, R. Ksouri, K. Chaieb, N. Karray-Bouraoui, N. Trabelsi, M. Boulaaba and C. Abdelly, “Phenolic composition of Cynara cardunculus L. organs, and their Biological Activities”, C. R. Biol., Vol. 331, 372–379, 2008.
 Y. Gong, Z. Q. Hou, Y. X. Gao, Y. S. Xue, X. Liu and G. M. Liu, “Optimization of extraction parameters of bioactive components from defatted Marigold (Tagetes erecta L.) residue using response surface methodology”, Food Bioprod. Process., Vol. 90, pp. 9–16, 2012.
 T. Guo, L. Wei, J. Sun, C. L. Hou and L. Fan, “Antioxidant activities of extract and fractions from Tuber indicum Cooke & Massee”, Food Chem., Vol. 127, pp. 1634–1640 2011.
 J. M. C. Gutteridge, “Ferrous-salt-promoted damage to deoxyribose and benzoate: The Increased effectiveness of hydroxyl-radical scavenger in the presence of EDTA”, Biochem. J., Vol. 243, pp. 709–714, 1987.
 S. Hemwimon, P. Pavasant and A. Shotipruk, “Microwave-assisted extraction of antioxidative anthraquinones from roots of morinda citrifolia”, Sep. Purif. Techn., Vol. 54, pp. 44–50, 2007.
 M. Ismail, A. A. Mariod, R. M. Ibrahim and N. Ismail, “Antioxidant activities of phenolic rich fractions (PRFs) obtained from black mahlab (Monechma ciliatum) and white mahlab (Prunus mahaleb) seedcakes”, Food Chem., Vol. 118, pp. 120–127, 2010.
 A. Kumaran and J. R. Karunakaran, “Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus”, Food Chem., Vol. 97, pp. 109–114, 2006.
 J. Li, Y. G. Zu, Y. J. Fu, Y. C. Yang, S. M. Li, Z. N. Li and M. Wink, “Optimization of microwave-assisted extraction of triterpene saponins from defatted residue of yellow horn (Xanthoceras sorbifolia Bunge.) kernel and evaluation of its antioxidant activity”, Innov. Food Sci. Emerg., Vol. 11, pp. 637–643, 2010.
 W. Liu, Y. J. Fu, Y. G. Zu, M. H. Tong, N. Wu, X. L. Liu and S. Zhang, “Supercritical carbon dioxide extraction of seed oil from Opuntia dillenii Haw and its antioxidant activity”, Food Chem., Vol. 114, pp.334–339, 2009. [Online]. Available: https://doi.org/10.1016/j.foodchem.2017.06.150.
 Y. Liu, X. M. Sun and B. H. Li, “Adsorption of Hg2+ and Cd2+ by ethylenediamine modified peanut shells”, Carbohydr. Polym., Vol. 81, pp. 335–339, 2010.
 Y. Q. Ma, J. C. Chen, D. H. Liu and X. Q. Ye, “Simultaneous extraction of phenolic compounds of citrus peel extracts, Effect of ultrasound”, Ultrason. Sonochem., Vol. 16, pp. 57-62, 2009. [Online]. Available:https://doi.org/10.1016/j.foodchem.2017.06.150.
 C. E. Missang, P. Massiot, A. Baronand, J. F. Drilleau, “Effect of oxidative browning of apple pulp on the chemical and enzymatic extraction of cell wall polysaccharides”, Carbohydr.Polym. Vol. 20, pp. 131–138, 1993.
 A. Moure, D. Franco, J. Sineiro, H. Dominguez, M. J. Nunezand J. M. Lema, “Evaluation of extracts from Gevuina hulls as antioxidants”, J. Agric. Food Chem., Vol. 48, pp. 3890–3897, 2000.
 L. Quanhong and F. Caili, “Application of response surface methodology for extraction optimization of germinant pumpkin seeds protein”, Food Chemistry, Vol. 92, pp. 701-706, 2005.
 E. M. Silva, H. Rogez and Y. Larondelle, “Optimization of extraction of phenolics from Inga edulis leaves using response surface methodology”, Sep. Purif. Techn., Vol. 55, pp. 381–3, 2007
 W. Wang, C. M. Xu and X. L. Cao, “Optimization of microwave-assisted extraction of polysaccharide from Morus alba L. by response surface methodology”, J. Beijing Technol.Bus. Univ. (Nat. Sci. Ed.) Vol. 27, No. 4, pp. 6–9, 2009.
 T. Xu and X. Q. Liu, “Peanut shell activated carbon: characterization, surface modification and adsorption of Pb2+ from aqueous solution”, Chin. J. Chem. Eng., Vol. 16, pp. 401–406, 2008.
 C. Zou, Y. M. Du, Y. Li, J. H.Yang, T. Feng, L. Zhang and J. F. Kennedy, “Preparation of lacquer polysaccharide sulfates and their antioxidant activity in vitro”, Carbohydr. Polym., Vol. 73, pp. 322–331, 2008.