Preliminary Phytochemical Tests and Antioxidant Potential of Catharanthus Roseus ExtractsAuthor : P. Ethalsha, P. Prema Kumari and K. Mercy Salima
Volume 4 No.2 July-December 2015 pp 41-43
Periwinkle (nayantara) is the common name for a pair of perennial flowering shrubs belonging to the Apocynaceae family. The herb has been used for centuries to treat a variety of ailments and was a favourite ingredient of magical charms it was in the middle ages. The latin name for this herb is Catharanthusroseus, but it was classified as Vincarosea, and is still called by that name in some of the herbal literature. The present study explores the various phytochemicals present in the plant so as to substantiate its antioxidant property and also shows the several phytoconstituents that contribute to its various medicinal properties. Preliminary phytochemical screening of the crude extracts revealed the presence of alkaloids,flavanoids, tannins, saponins and phenolics. During DPPH assay of methanol extract of aerial part of Catharanthusroseusshows highest antioxidant activity of 92.04% in 200(μg/ml). The results confirm that aerial part of Catharanthusroseuscan be used as source of drugs to fight infections caused by susceptible bacteria.
DPPH Assay, Phytochemicals, Antioxidant, C.roseus
 S. K. Uniyal, K. N. Singh, P. Jamwal, and B. Lal, Traditional use of medicinal plants, 2006.
 P. K. Lai and J. Roy, “Antimicrobial and Chemopreventive properties of herbs and spices”, Curr.Med.Chem., pp. 451-1460, 2004.
 S. Padua, J. K. Johnson and M. Massiot, Res.J. Med. & Med. Sci., Vol. 29, pp.147, 1999.
 C. Berrier, J. C. Jacquesy, M. P. Jouannetaud and A. Renoux, “Hydroxylation and bromination of indolines and tetrahydro- quinolines in superacids”, New J. Chem, Vol. 11, pp. 605, 1987.
 C. J. Barnett, G. J. Cullinan, K. Gerzon, R. C. Hoying, W. E. Jones, W. M. Newlon, G. A. Poore, “Structure-activity relation-ships of dimeric Catharanthus alkaloids. 1. Deacetylvinblastine amide (vindesine) sulfate”, J Med Chem, Vol. 21,pp. 88, 1978
 J. T. Basker, R. P. Barris, and B. Carte, “Natural products drug discovery”, New perspective on international collaboration, J.Net, Vol. 58, pp. 1325-1357, 1995.
 H. L. Pearce, “Medicinal chemistry of bisindole alkaloids from Catharanthus”, In: Brossi A, Su€nessM(eds) The alkaloids ± antitumor bisindole alkaloids from Catharanthusroseus (L.). Academic Press, San Diego, pp. 145, 1990.
 Mohammed Ibrahim, Syedamughramehjabeen and Mangamoor Lakshmi Narsu, International of Pharmaceutical Applications, Vol. 2, pp. 165-173, 2011.
 ShivanandaNayak, Online journal of Biological Sciences, Vol 6, No. 2, pp.40-44, 2006.
 S. T. Chang, J. H. Wu, S. Y. Wang, P. L. Kang, N. S. Yang, and L. F. Shyur, “Antioxidant activity of extracts from Acacia confusa bark and heartwood”, Journal of Agricultural and Food Chemistry, Vol. 49, pp. 3420–3424, 2001.
 J. B. Harborne, Phytochemical Methods: A guide to modern techniques of plant analysis, 3rd edn. Chap-man and Hall, New York, pp. 1–150, 1998.
 A. M. El-Mahmood, J. H. Doughari, and F. J. Chanji, “In-vitro antibacterial activities of crude extracts of Nauclealatifolia and Daniellaoliveri”, Sci. Res.Essay, Vol. 3, No.3, pp. 102-105, 2008.
 J. A. Duke, and E. S. Ayensu, “Medicinal plants of China”, Algonae, Mich. Reference Publications 2, Medicinal plants of the World, No. 4, 1985.
 A. A. Nguyi, “Tannins of some Nigerian flora”, Niger. J. Biotechnol, Vol. 6, pp. 221-226, 1988.